Apparatus for attaching a labeled probe and/or antibody to macromolecules
US5425917A · kind A · utility
Inventor
Key dates
| Filing date | May 13, 1994 |
| Grant date | Jun 20, 1995 |
| Priority date | — |
| Expiry date | May 13, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44717
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus and method for attaching a labeled probe to macromolecules such as DNA/RNA fragments or proteins attached to a membrane utilizes a flexible bag having an inlet and an outlet port. Reagents including the probe to which the membrane is to be exposed during the labeling process are stored in reservoirs. Pumps are provided to selectively transfer reagents from reservoirs to the inlet port and for selectively evacuating the bag via the outlet port so that reagents from the individual reservoirs can be sequentially supplied to or removed from the bag while the membrane is positioned therein. One or more rollers are arranged to roll back and forth over the top of the bag to uniformly expose the membrane to the reagent within the bag.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.