Extraction methods for preparing thromboplastin reagents
US5426031A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 25, 1993 |
| Grant date | Jun 20, 1995 |
| Priority date | — |
| Expiry date | May 25, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/7454
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The Prothrombin Time (PT) is used as a screening test for blood coagulation factor deficiencies and for monitoring oral anti-coagulant therapy using coumadin. Thromboplastin reagents activate the extrinsic pathway of coagulation and are the basis for the Prothrombin Time (PT) test. This invention describes the use of barium sulfate and chaotropic agents, and nonionic detergents, for the extraction of sensitive thromboplastin reagents from tissue. Extraction with sodium thiocyanate alone also greatly enhances thromboplastin sensitivity. This invention should be useful for all thromboplastins and will improve their sensitivity for all PT-based tests and specific assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.