Direct molecular cloning of primer extended DNA containing an alkane diol
US5426039A · kind A · utility
94Cited by
2References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 8, 1993 |
| Grant date | Jun 20, 1995 |
| Priority date | — |
| Expiry date | Sep 8, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a method of cloning DNA produced by primer extension including PCR amplified, reverse transcriptase-generated or primer extended synthetic DNA. Specifically, it relates to a method in which alkane diol residue containing oligonucleotide primers are incorporated into DNA by primer extension followed by direct cloning of the target DNA. Following transformation, the host excises the alkane diol residue with its endogenous DNA repair machinery.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.