DNA encoding cytokine-induced protein, TSG-14
US5426181A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 14, 1992 |
| Grant date | Jun 20, 1995 |
| Priority date | — |
| Expiry date | Aug 14, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Pleiotropic pro-inflammatory cytokines, such as TNF and IL-1, induce expression of a polypeptide molecule, termed TSG-14, in connective tissue cells. The TSG-14 polypeptide and functional derivatives thereof, DNA coding therefor, expression vehicles, such as a plasmids, and host cells transformed or transfected with the DNA molecule, and methods for producing the polypeptide and the DNA are provided. Antibodies specific for the TSG-14 polypeptide are disclosed, as is a method for detecting the presence of TSG-14 polypeptide in a biological sample, using the antibody or another molecule capable of binding to TSG-14 such as hyaluronic acid. A method for detecting the presence of nucleic acid encoding a normal or mutant TSG-14 polypeptide, a method for measuring induction of expression of TSG-14 in a cell using either nucleic acid hybridization or immunoassay, a method for identifying a compound capable of inducing the expression of TSG-14 in a cell, and a method for measuring the ability of a cell to respond to TNF are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.