Process for the enzymatic cleavage of recombinant proteins using IgA proteases

US5427927A · kind A · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Thomas F. Meyer · Tübingen, DE
• Johannes Pohlner · Tübingen, DE
• Gunter Schumacher · Altenkirchen, DE
• Carola Dony · Starnberg, DE

Key dates

• Filing date: Aug 30, 1992
• Grant date: Jun 27, 1995
• Expiry date: Aug 30, 2012

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

For the enzymatic cleavage of fusion proteins and for the isolation of deed components of these fusion proteins PA1 (1) a junction region, in which two components of the fusion protein are joined together, is modified by means of genetic engineering so that at least one IgA protease recognition site with the amino acid sequence Y-Pro.!.X-Pro is formed in this junction region, in which X can be any amino acid and Y can be one or several arbitrary amino acids, PA1 (2) the fusion protein which results from step (1) is cleaved by IgA protease at the position in the recognition site marked with .!. and PA1 (3) after the cleavage one or several desired components of the fusion protein are isolated.