Octopine T-DNA promoters
US5428147A · kind A · utility
43Cited by
1References
21Claims
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Inventors
Key dates
| Filing date | Jul 13, 1993 |
| Grant date | Jun 27, 1995 |
| Priority date | — |
| Expiry date | Jul 13, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/146
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.