Antibody-mediated juxtaposition of reactive moieties

US5429936A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Peter G. Schultz · Village of La Jolla, US
• Paul A. Bartlett · Kensington, US

Key dates

• Filing date: Apr 21, 1989
• Grant date: Jul 4, 1995
• Expiry date: Apr 21, 2009

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/12
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A variety of chemical reactions are rate enhanced by the use of antibodies whose antibody binding sites are complementary to the reactant orientations or transition states which lead to the desired product. The reactions include pericyclic reactions such as Claisen and Cope rearrangements and Diels Alder reactions, peptide bond hydrolysis reactions, peptide fragment ligations, lactonizations and cyclic peptide syntheses, glycosylations, aldol additions, nucleoside syntheses and transesterification reactions. Haptens which are stable analogues of unstable transition states, or which are analogues of desired products, in some cases with leaving groups still attached for purposes of avoidance of product inhibition, are used to generate the antibodies.