Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5432272A · kind A · utility
823Cited by
2References
7Claims
0Family size
Inventor
Key dates
| Filing date | Oct 9, 1990 |
| Grant date | Jul 11, 1995 |
| Priority date | — |
| Expiry date | Oct 9, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure describes a method for incorporating into double stranded DNA and RNA base pairs composed of pairing units that fit the Watson-Crick geometry in that they involve a monocyclic six membered ring pairing with a fused bicyclic heterocyclic ring system composed of a five member ring fused with a six membered ring, with the orientation of the heterocycles with respect to each other and with respect to the backbone chain analogous to that found in DNA and RNA, but with a pattern of hydrogen bonds holding the base pair together different from that found in the AT and GC base pairs (a "non-standard base pair").
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.