Complementation assay for drug screening
US5434052A · kind A · utility
9Cited by
9References
8Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jul 21, 1993 |
| Grant date | Jul 18, 1995 |
| Priority date | — |
| Expiry date | Jul 21, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Compounds are evaluated for their binding to naturally occurring receptors, by employing the natural ligand conjugated to an enzyme donor fragment of .beta.-galactosidase for competing with the sample compound for the natural acceptor binding site or in the absence of competition where the sample compound binds to an allosteric site. By adding the enzyme acceptor fragment of the .beta.-galactosidase and substrate, the binding affinity of the sample compound may be evaluated as a measure of agonist or antagonist capability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.