Patent · US Expired

Devices for determining hydrolase activity through its hydrolytic release of an immobilized indicator enzyme

US5434054A · kind A · utility

3Cited by

15References

3Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Klaus Pollmann · Neulußheim, DE
Helmut Freitag · Indianapolis, US
Anselm Rothe · Birkenau, DE

Key dates

Filing date	May 27, 1993
Grant date	Jul 18, 1995
Priority date	—
Expiry date	May 27, 2013

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/97
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Subject matter of the invention is a new method of detecting a substance with hydrolase activity in a sample, characterized in that the sample is brought into contact with an indicator enzyme, which is covalently bound to an insoluble carrier material and can be rendered soluble by the hydrolase activity, in that the cleaved off indicator is separated and in that its enzymatic activity is determined and test means for its implementation.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.