Process for the production and renaturation of recombinant, biologically active, eukaryotic alkaline phosphatase

US5434067A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Uwe Michaelis · Darmstadt, DE
• Rainer Rudolph · Waldshut-Tiengen, DE
• Michael Jarsch · Bad Heilbrunn, DE
• Erhard Kopetzki · Penzberg, DE
• Helmut Burtscher · Habach, DE
• Gether Schumacher · Bernried, DE

Key dates

• Filing date: Jul 30, 1993
• Grant date: Jul 18, 1995
• Expiry date: Jul 30, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In a process for the production of recombinant, biologically active, eukaryotic alkaline phosphatase a DNA sequence coding for a eukaryotic alkaline phosphatase is expressed in a prokaryotic host cell and the expression product is obtained in an active form by cell lysis, solubilization under denaturing conditions and subsequent renaturation. In this process the renaturation step is carried out in the presence of one or several stabilizing agents.