Method for cloning and producing the BglII restriction endonuclease and modification methylase
US5434068A · kind A · utility
2Cited by
0References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 9, 1993 |
| Grant date | Jul 18, 1995 |
| Priority date | — |
| Expiry date | Apr 9, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the BglII endonuclease by: 1) introducing the restriction endonuclease gene from Bacillus globigii into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the BglII restriction endonuclease activity; and 3) purifying the BglII restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the BglII restriction endonuclease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.