Method for preparing an aqueous solution enriched in both EG-III & xylanase using a low molecular weight alcohol and an organic salt
US5434072A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 18, 1994 |
| Grant date | Jul 18, 1995 |
| Priority date | — |
| Expiry date | Apr 18, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for preparing an aqueous solution enriched in xylanase only, EG III only and both EG III and xylanase from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The methods involve adding an amount of a low molecular weight alcohol and an organic salt to an aqueous mixture containing cellulase proteins under conditions wherein substantially all of the cellulase proteins other than EG III and xylanase are precipitated out of the aqueous mixture. The methods can then involve adding an inorganic salt to the supernate produced in the previous step so as to form a second precipitate and a second supernate and then finally collecting either the second precipitate from the second supernate to obtain a precipitate enriched in xylanase or the second supernate from the second precipitate to obtain a supernate enriched in EG III.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.