Assay methods and compositions for detecting and evaluating the intracellular transduction of an extracellular signal
US5436128A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 27, 1993 |
| Grant date | Jul 25, 1995 |
| Priority date | — |
| Expiry date | Jan 27, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Transcription based assays that identify extracellular signals that modulate the activity of cell surface proteins are provided. Extracellular signals are identified by measuring the amount of transcription of a reporter gene in a recombinant cell that expresses the cell surface protein and contains DNA encoding the reporter gene under the transcriptional control of a promoter that is regulated, directly or indirectly, by the cell surface protein. The assays, provide a means for identifying potential pharmaceutical compounds that can be used to treat disease by virtue of their agonistic or antagonistic effects on the cell surface protein. Recombinant cells that express cell surface receptors and that contain reporter gene constructs that include transcriptional regulatory elements that are responsive to the activity of the cell surface receptors are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.