Multi-domain DNA ligands bound to a solid matrix for protein and nucleic acid affinity chromatography and processing of solid-phase DNA
US5437976A · kind A · utility
32Cited by
6References
16Claims
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Key dates
| Filing date | Aug 8, 1991 |
| Grant date | Aug 1, 1995 |
| Priority date | — |
| Expiry date | Aug 8, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a multi-domain oligonucleotide ligand having multiple functions conferred by each of the domains. The ligand is bound to a solid phase matrix for use in both mRNA-affinity chromatography and as a priming matrix for generating matrix-bound cDNA from the mRNA bound to the matrix. The resultant cDNA was tested and found to function as a solid-phase template for PCR. This solid-phase template approach to PCR is demonstrated with oligo-d(T) paper. The functional domains are defined by polynucleotide sequences on the ligand to confer the different functions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.