DNA probe signal amplification
US5437977A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 29, 1992 |
| Grant date | Aug 1, 1995 |
| Priority date | — |
| Expiry date | May 29, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/682
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for amplifying a signal during the detection of target nucleic acid molecules utilizes a primary oligonucleotide probe that binds to a bridging nucleic acid molecule. The bridging molecule hybridizes to a first developer nucleic acid molecule. Each first developer molecule comprises: (a) a first branch having a sequence of at least two different nucleotides and at least six total nucleotides complementary to a sequence of a first branch of a second developer molecule; (b) a second branch comprising a sequence of at least two different nucleotides and at least six total nucleotides complementary to a sequence of a second branch of the second developer molecule; and (c) a detectable label. In the method, the bridging molecule binds to the primary probe and hybridizes to the first developer molecule; the bound first developer molecule hybridizes to the second developer molecule to form a developer chain; additional first and second developer molecules are added to the chain; and the labeled developer molecules in the developer chain are detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.