Method of preparation of purified alkaline protease
US5439817A · kind A · utility
16Cited by
2References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 21, 1993 |
| Grant date | Aug 8, 1995 |
| Priority date | — |
| Expiry date | Jan 21, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/816
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.