Methods and apparatus for real-time monitoring, measurement and control of electroosmotic flow
US5441613A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 3, 1993 |
| Grant date | Aug 15, 1995 |
| Priority date | — |
| Expiry date | Dec 3, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44752
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In capillary electrophoresis systems, real-time monitoring and measurement of the electroosmotic flow through a separation capillary is accomplished by coupling the outlet of the separation capillary to an electrically-conductive junction. In one embodiment, this junction is an ion-impermeable or an ion-exchange membrane unit that preferentially exchanges ions having a charge opposite to analyte ions of interest. Within a downstream region of the junction, all axial incremental voltage from the electroosmotic voltage source is terminated, which ensures that downstream electrolyte ion movement is passive, due to active flow created upstream when an incremental axial voltage existed. Upstream electrolyte ion flux is proportional to C.sub.1 .multidot.(.mu..sub.e +.mu..sub.eo), where C.sub.1 is the upstream concentration of the electrolyte ion of interest, .mu..sub.e is the electrolyte electrophoretic mobility, and .mu..sub.eo is the electroosmotic mobility. Downstream, the flux is proportional to C.sub.2 .multidot..mu..sub.eo, where C.sub.2 is the downstream concentration of the electrolyte ion of interest. The fluxes are equal, whereupon .mu..sub.eo .apprxeq.C.sub.1 .multidot..mu..su…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.