Method for preparing modified subtilisins
US5441882A · kind A · utility
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7Claims
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Key dates
| Filing date | May 9, 1990 |
| Grant date | Aug 15, 1995 |
| Priority date | — |
| Expiry date | May 9, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/07
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There are described methods for making mutant subtilisins, the methods comprising obtaining a DNA fragment from a Bacillus subtilisin and introducing a mutation into the fragment by substituting at least one amino acid, transforming a suitable host cell with the mutated DNA, recovering a mutant subtilisin and screening the mutant subtilisin for certain altered enzymatic properties.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.