Electrobiochemical analytical method and electrodes

US5443701A · kind A · utility

Assignee

• YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD. · IL

Inventors

• Itamar Willner · Mevaseret Tsiyon, IL
• Evgeny Katz · Jerusalem, IL
• Azalia Riklin · Jerusalem, IL
• Ron Kasher · Jerusalem, IL
• Benjamin Shoham · Jerusalem, IL

Key dates

• Filing date: Aug 23, 1993
• Grant date: Aug 22, 1995
• Expiry date: Aug 23, 2013

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/817
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An analytical method for determining the presence or concentration of an analyte in a liquid medium is provided in which electrons are transferred from an electrode material of an electrode to the redox center of an enzyme, by the mediation of an electron mediator, whereby in the presence of an analyte the enzyme calalyzed a redox reaction in which the analyte is converted into a product. By measuring either the concentration or the product or the charge which flows in this process, the presence and/or concentration of the analyte in the medium is determined. Either or both of the enzyme and the electron mediator are immobilized on the electrode.