Method of culturing lymphocytes and method of treatment using such lymphocytes
US5443983A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 21, 1988 |
| Grant date | Aug 22, 1995 |
| Priority date | — |
| Expiry date | Mar 21, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/515
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
In accordance with the present invention, a method of culturing lymphocytes is disclosed in which the lymphocytes are cultured in the presence of interleukin-2 (IL-2) and an antibody to a lymphocyte surface receptor, preferably an antibody to a lymphocyte T3 surface receptor. Preferably, the antibody is monoclonal. Cells cultured with anti-CD3 maintain their LAK activity as they increase in number. The cells can also be cultured in the presence of an additional lymphokine to obtain additional LAK activity. Methods of medical treatment are also disclosed in which cells cultured in accordance with the culturing methods of the present invention are introduced into the individual to be treated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.