Amplification assay for hydrolase enzymes
US5445942A · kind A · utility
9Cited by
2References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 13, 1993 |
| Grant date | Aug 29, 1995 |
| Priority date | — |
| Expiry date | Jan 13, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Hydrolase enzymes are sensitively determined using novel substituted FAD substrates. The substituted FAD substrates are hydrolysed to FAD by the enzyme to be detected. The FAD is then combined with an apoenzyme to form a holoenzyme which is used to initiate a reaction that leads to a detectable product. When the hydrolase to be detected is phosphatase, the novel substrate is a phosphorylated derivative of FAD. A suitable apoenzyme is apo-glucose oxidase which provides exceptional sensitivity. Apo-D-amino acid oxidase, which is suitable for use in a "single pot" assay system, can also be used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.