Capture method for cell nucei using a DNA mesh
US5447864A · kind A · utility
24Cited by
1References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 14, 1993 |
| Grant date | Sep 5, 1995 |
| Priority date | — |
| Expiry date | Sep 14, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/255
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of separating cell nuclei from cells comprises: treating a fluid containing whole cells so as to selectively lyse the cytoplasmic membrane together with a small proportion of the nuclear membranes but leaving a large proportion of the cell nuclei intact; applying the treated fluid to a membrane whereby a mesh of DNA from the lysed nuclei is formed on the surface and captures intact cell nuclei. A device for use in the method is described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.