Assay for the quantitative determination of substrates capable of undergoing enzymatic hydrolysis to release long-chain fatty acids
US5449607A · kind A · utility
14Cited by
5References
10Claims
0Family size
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Key dates
| Filing date | Aug 18, 1993 |
| Grant date | Sep 12, 1995 |
| Priority date | — |
| Expiry date | Aug 18, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/044
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of quantitative assay for a substrate such as triglyceride capable of undergoing enzymatic hydrolysis to release long chain fatty acids in an albumin-containing clinical sample comprises incubating the albumin-containing clinical sample with an enzyme such as lipase which acts upon the substrate to be assayed under conditions effective to release fatty acid therefrom, causing the fatty acid thus released to bind to a fatty acid binding protein (FABP) and assaying the binding of the fatty acid to the FABP.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.