Reacting an enzyme in a non-aqueous solvent by adding a lyophilizate of enzyme and salt to the solvent
US5449613A · kind A · utility
25Cited by
0References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 1, 1994 |
| Grant date | Sep 12, 1995 |
| Priority date | — |
| Expiry date | Mar 1, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/96
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for reacting an enzyme in a non-aqueous organic solvent is disclosed. The method comprises preparing a lyophilizate of a salt which activates the enzyme and an enzyme wherein the lyophilizate contains a weight ratio of salt to enzyme of at least 60% salt sufficient to activate the enzyme in an organic solvent. The method then calls for dispersion of the lyophilizate in a non-aqueous organic solvent in the presence of a substrate for the enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.