Non-nucleoside 1,3-diol reagents for labeling synthetic oligonucleotides
US5451463A · kind A · utility
248Cited by
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15Claims
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Key dates
| Filing date | Jun 3, 1993 |
| Grant date | Sep 19, 1995 |
| Priority date | — |
| Expiry date | Jun 3, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2982
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A trifunctional reagent possessing a primary hydroxyl, a secondary hydroxyl, and a primary amino group. This reagent is useful in solid phase oligonucleotide synthesis for the convenient labeling of the 3'-terminus. The secondary hydroxyl may be an phosphoramidite derivative permitting the attachment to the solid phase support. The reporter molecule may be attached to the trifunctional molecule prior to the completion of the oligonucleotide synthesis or after the oligonucleotide is cleaved from the support. A preferred species of this trifunctional molecule is shown below.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.