Restriction amplification assay
US5451502A · kind A · utility
10Cited by
10References
44Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Aug 31, 1993 |
| Grant date | Sep 19, 1995 |
| Priority date | — |
| Expiry date | Aug 31, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/708
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method, reagent and kit for the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.