Patent · US Expired

Method and monoclonal antibodies for vitamin B12 determination

US5451508A · kind A · utility

3Cited by

4References

11Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Nicholas Hoyle · Tutzing, DE
Gunter Pappert · Tutzing, DE
Michael Grol · Feldafing, DE
Christa Hubner-Parajsz · Tutzing, DE

Key dates

Filing date	Oct 15, 1993
Grant date	Sep 19, 1995
Priority date	—
Expiry date	Oct 15, 2013

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S436/815
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

For the determination of vitamin B12 a sample solution is incubated with at least two receptors R.sub.1 and R.sub.2 of which R.sub.1 mediates the binding to the solid phase and R.sub.2 is labelled, whereby a receptor is used as one of the receptors R.sub.1 or R.sub.2 which contains a monoclonal antibody capable of specific binding to B12 that has an affinity constant of at least 5.times.10.sup.9 l/mol and a receptor is used as the other receptor R.sub.1 or R.sub.2 which contains B12 or an analogue thereof, the two phases are separated and the label is measured in one of the two phases.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.