D-arabinitol dehydrogenase from Candida tropicalis ATCC 750 or Candida shehatae
US5451517A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 3, 1995 |
| Grant date | Sep 19, 1995 |
| Priority date | — |
| Expiry date | Mar 3, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/924
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is substantially free of other enzymes capable of oxidizing D-mannitol. Also disclosed are methods for determining D-arabinitol. In one embodiment the method comprises the steps of providing in combination (1) a medium suspected of containing D-arabinitol and (2) a D-arabinitol dehydrogenase enzyme and examining the medium for the product of the oxidation of the D-arabinitol. The enzyme utilized is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol. Kits for conducting the present method are also disclosed. The D-arabinitol dehydrogenase in purified form has all of the characteristics of a D-arabinitol dehydrogenase which is obtainable from Candida tropicalis ATCC 750 or Candida shehatae.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.