Patent · US Expired

Calibrated spectrographic imaging

US5452723A · kind A · utility

221Cited by
24References
22Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 24, 1992
Grant dateSep 26, 1995
Priority date
Expiry dateJul 24, 2012

Classification

  • Technology area (CPC A)Human Necessities
  • CPC primaryA61B2562/146
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

The present application is directed to the use of photon migration analysis to provide a method of analyzing the diffuse reflectance, fluorescence, Raman or other types of spectra obtained from tissue. This procedure provides a means for processing spectral data such that the distortion in fluorescence spectra, for example, caused by the interplay of a variety of factors such as scattering, absorption, geometry and boundary conditions, can be precisely removed simply by measuring the diffuse reflectance spectrum as well as a second selected spectrum, such as fluorescence, and adjust the spectrum with the reflectance spectrum as described herein. By this procedure, the sample-to-sample variability is minimized. The intrinsic spectrum extracted by this procedure can be easily deconvoluted and provide quantitative information about the physicochemical composition of tissue. Analytical procedures for clinical diagnosis have been developed based on this method.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.