Pluripotential embryonic stem cells and methods of making same
US5453357A · kind A · utility
202Cited by
2References
14Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 8, 1992 |
| Grant date | Sep 26, 1995 |
| Priority date | — |
| Expiry date | Oct 8, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a non-mouse pluripotential embryonic stem cell which can: PA1 (a) be maintained on feeder layers for at least 20 passages; and PA1 (b) give rise to embryoid bodies and multiple differentiated cell phenotypes in monolayer culture. The invention further provides a method of making a pluripotential embryonic stem cell comprising administering a growth enhancing amount of basic fibroblast growth factor, leukemia inhibitory factor, membrane associated steel factor, and soluble steel factor to primordial germ cells under cell growth conditions, thereby making a pluripotential embryonic stem cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.