Recombinant poxvirus host range selection system
US5453364A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 5, 1993 |
| Grant date | Sep 26, 1995 |
| Priority date | — |
| Expiry date | Aug 5, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/20122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
What is described is a modified recombinant virus for expressing a gene product in a host. The modified recombinant virus has host range genes deleted therefrom so that the virus has restricted replication in the host. The modified recombinant virus also contains DNA which codes for and expresses the gene product in the host even with restricted replication of the virus in the host. The modified recombinant virus is used in a method for expressing a gene product in a host or in a cell cultured in vitro, and in a vaccine for inducing an immunological response in a host inoculated with the vaccine. What is also described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conditional lethal mutant (host range) of poxviruses. A deletion/recombinant mutant of the vaccinia virus was generated which is capable of plaquing on primary chick embryo fibroblasts and two monkey cell lines (BSC-40 or VERO) but is defective in replication in the human cell line MRC-5. Insertion of the host range gene into the deletion/recombinant restores the ability for growth on MRC-5 cells. A series of plasm…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.