Stabilized enzymes and process for preparing them

US5453372A · kind A · utility

Assignees

• Solvay Enzymes GmbH & Co. KG · DE
• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• Roman Vetter · Burgdorf, DE
• Ingo Muecke · Barsinghausen, DE
• Detlef Wilke · Wrisbergholzen, DE

Key dates

• Filing date: Jul 27, 1992
• Grant date: Sep 26, 1995
• Expiry date: Jul 27, 2012

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/54
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.