Trigonopsis transformant producing D-amino acid oxidase
US5453374A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 23, 1993 |
| Grant date | Sep 26, 1995 |
| Priority date | — |
| Expiry date | Jul 23, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/911
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A Trigonopsis variabilis transformed with a recombinant DNA containing a D-amino acid oxidase gene capable of expressing in Trigonopsis variabilis is provided. A process for transforming Trigonopsis variabilis and a process for preparing 7-.beta.-(5-carboxy-5-oxopenetaneamide)cephalosporanic acid by using a transformant of Trigonopsis variabilis are also provided. The transformant of Trigonopsis variabilis shows high DAO activity and low activity of an esterase which interferes with the preparation of cephalosporin C. Accordingly, the Trigonopsis variabilis of the present invention enables one to produce cephalosporin C. Moreover, the Trigonopsis variabilis of the present invention can be used for the preparation or cephalosporin C merely by treating the cells with toluene so that large scale use is practical.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.