N-heteroaromatic ion and iminium ion substituted cyanine dyes for use as fluorescence labels
US5453505A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 30, 1994 |
| Grant date | Sep 26, 1995 |
| Priority date | — |
| Expiry date | Jun 30, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/802
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to iminium ion substituted cyanine dyes having a fluoresence absorbance of between about 500 and 900 nm, a reduced tendency to aggregate and enhanced photostability. The cyanine dyes of the present invention are represented by the formula ##STR1## wherein n is 0, 1, 2 or 3; PA1 m is 0, 1, 2 or 3; PA1 R.sub.1 and R.sub.2 are taken together to form an aromatic ring or a fused polycyclic aromatic ring; PA1 R.sub.3 and R.sub.4 are taken together to form an aromatic ring or a fused polycyclic aromatic ring; PA1 R.sub.5 and R.sub.6 are independently selected from the group consisting of (CH.sub.2).sub.p X where p is 1-18 and X is a functional group that reacts with amino, hydroxyl and sulfhydryl nucleophiles; PA1 R.sub.7 and R.sub.8 are independently selected from the group consisting of hydrogen, C1-C10 alkyl groups and where R.sub.7 and R.sub.8 are taken together to form a five- or six- membered heterocyclic ring; PA1 R.sub.9 are each independently selected from the group consisting of hydrogen, alkyl and where more than one R.sub.9 are taken together to form a five- or six- membered ring; PA1 Y is selected from the group consisting of C(CH.sub.3).sub.2, S…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.