Patent · US Expired

Rapid thermal cycling device

US5455175A · kind A · utility

188Cited by
19References
12Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJan 10, 1994
Grant dateOct 3, 1995
Priority date
Expiry dateJan 10, 2014

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/809
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A thermal cycling device having a sample chamber whose temperature can be rapidly and accurately modulated over a range of temperatures needed to carry out a number of biological procedures, such a the DNA polymerase chain reaction. Biological samples are placed in glass microcapillary tubes and then located inside the sample chamber. A programmable controller regulates the temperature of the sample inside the sample chamber. Once a heating cycle is completed, the controller opens a door to the chamber for venting hot air out and cool ambient air is moved in. Temperature versus time profiles corresponding to optimum denaturation, annealing and elongation temperatures for amplification of DNA are achievable by the present invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.