Agarose compositions for nucleic acid sequencing

US5455344A · kind A · utility

Assignee

• FMC Corporation · US

Inventors

• David L. Harper · Rockland, US
• Jonathan H. Morgan · Camden, US
• Samuel Nochumson · Rockland, US
• Mikhail V. Ostrovsky · Rockland, US
• Donald W. Renn · Glen Cove, US
• William C. Snow · Rockland, US

Key dates

• Filing date: Sep 3, 1993
• Grant date: Oct 3, 1995
• Expiry date: Sep 3, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08L5/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A nucleic acid sequencing gel comprising 2 to 10% of a gelling polysaccharide, preferably agarose; a denaturing agent, a non-gelling additive, preferably glycerol, and electrophoretic buffers. This composition is capable of resolving nucleic acid fragments having at least 50 bases and which differ from each other by at least one base.