Substituted thiazoline-dioxetan substrates, process for the production and use

US5455357A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Rupert Herrmann · Weilheim, DE
• Hans-Peter Josel · Weilheim, DE
• Christian Klein · Iffeldorf, DE
• Dieter Heindl · Munich, DE

Key dates

• Filing date: Nov 30, 1993
• Grant date: Oct 3, 1995
• Expiry date: Nov 30, 2013

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/582
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Compounds of the general formula III, ##STR1## in which R represents ##STR2## and in which R.sub.1 and R.sub.2 are the same or different and represent hydrogen or straight-chained or branched lower alkyl C.sub.1 -C.sub.6, and X represents a cleavable group, at least one of the groups R.sub.4 or R.sub.5 represents a group that stabilizes the dioxetan structure and at most one of the groups R.sub.4 or R.sub.5 represents hydrogen. These compounds can be used as substrates in immunological assays and in DNA diagnostics using activating agents for the formation of colour.