Enzyme amplified, complex linked, competitive and non-competitive assays for the detection of metal ions
US5459040A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 22, 1993 |
| Grant date | Oct 17, 1995 |
| Priority date | — |
| Expiry date | Nov 22, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/13
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods of assaying for the presence or amount of a metal ion in a sample suspected of containing such ions. In one aspect, an enzyme amplified sandwich assay is provided which relies upon the ability of the analyte (metal ion) to form a complex with two complexing agents (chelators). In this assay, the first sandwich chelator is immobilized on a solid support, while the second sandwich chelator is linked to a reporter group (e.g., an enzyme). This assay combines the specific recognition of the analyte by the first and second sandwich chelators with the great signal amplification offered by the reporter group (e.g., enzyme). In another aspect, a competitive assay is provided that relies on the competitive inhibition of complex formation between the coating ligand (i.e., the chelator attached to the solid support) and the organometallic compound attached to the reporter group (e.g., enzyme) by the metal ions of interest present in the sample. Using the methods of the present invention, hazardous metals, environmental pollutants, and other biomolecules can be selectively detected at ppb/ppt concentrations. More particularly, using the methods of the present invention, highly toxic me…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.