Methods and reagents for performing ion-capture digoxin assays

US5459078A · kind A · utility

Assignee

• Abbott Laboratories · US

Inventors

• Steven J. Kline · Grayslake, US
• Yi-Her Jou · Libertyville, US
• Stephen D. Stroupe · Libertyville, US
• Janina Adamczyk · Gurnee, US
• Daniel S. Berry · Libertyville, US
• Rosario M. Fico · Zion, US
• James J. Markese · Downers Grove, US

Key dates

• Filing date: Jun 9, 1993
• Grant date: Oct 17, 1995
• Expiry date: Jun 9, 2013

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/825
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention includes novel digoxin assays employing a capture reagent, involving a first binding member conjugated to a polymeric anion substance, and a solid phase material containing a reaction site comprising a polymeric cation substance having a nitrogen content of at least about two percent. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex.