Ion-capture assays using a specific binding member conjugated to carboxymethylamylose
US5459080A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 27, 1994 |
| Grant date | Oct 17, 1995 |
| Priority date | — |
| Expiry date | Jan 27, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/961
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention includes novel assays employing a capture reagent, involving a first specific binding member conjugated to a polymeric anion such as carboxymethylamylose, and a solid phase material containing a reaction site comprising a polymeric cation substance. A test sample suspected of containing the analyte of interest may be contacted with the capture reagent to form a charged capture reagent/analyte complex. The complex is then contacted to/ the oppositely charged solid phase to attract, attach, and immobilize the capture reagent/analyte complex. The use of carboxymethylamylose to prepare a suitably charged capture reagent provides a superior capture reagent that is capable of binding and retaining the analyte on the solid phase even in the presence of polyanionic non-specific binding blockers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.