Direct labeling of antibodies and other protein with metal ions
US5460785A · kind A · utility
44Cited by
19References
42Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 3, 1992 |
| Grant date | Oct 24, 1995 |
| Priority date | — |
| Expiry date | Jan 3, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/866
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Proteins containing one or more dtsulfide bonds or monosulfides are labeled with medically useful metal ions for use in diagnosis and treatment of a variety of pathologtc conditions. Labeling is accomplished with a variety of metals, including radiometals, by using a reducing agent to reduce the disulfide bonds to thiolate groups; excess reducing agent, reaction by-products and any impurities are removed; and, a source of Sn (II) agent is added to the thiolate-containing protein. The resulting product may be stored frozen or lyophilized, with labeling accomplished by the addition of the medically useful metal ions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.