Inverse linkage oligonucleotides for chemical and enzymatic processes
US5462854A · kind A · utility
92Cited by
7References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 19, 1993 |
| Grant date | Oct 31, 1995 |
| Priority date | — |
| Expiry date | Apr 19, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6862
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process are disclosed. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.