Dry multilayer analytical elements for assaying transaminases
US5462858A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 29, 1993 |
| Grant date | Oct 31, 1995 |
| Priority date | — |
| Expiry date | Dec 29, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A dry multilayer analytical element for assaying transaminases wherein the analytical element comprises: PA0 a) a transaminase substrate; PA0 b) reagents that generate pyruvate in the presence of a transaminase and the transaminase substrate; PA0 c) pyruvate oxidase and cofactors for pyruvate oxidase; and PA0 d) reagents that generate a color in proportion to a concentration of hydrogen peroxide; and wherein the analytical element has, in the following order, a spreading layer, a reagent layer, and a support wherein PA0 i) the spreading layer contains the transaminase substrate and a material selected from the group consisting of polymer beads, barium sulfate, titanium oxide, and mixtures thereof; PA0 ii) the reagent layer contains the pyruvate oxidase; and PA0 iii) both a hardened gelatin layer and a subbing layer separate the reagent layer from the spreading layer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.