Isolation of Hepatitis B surface antigen from transformed yeast cells
US5462863A · kind A · utility
11Cited by
6References
26Claims
0Family size
Assignee
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Key dates
| Filing date | Apr 28, 1994 |
| Grant date | Oct 31, 1995 |
| Priority date | — |
| Expiry date | Apr 28, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for recovering hepatitis B surface antigen protein from transformed yeast cells including the steps of (i) obtaining an aqueous homogenate of the yeast cells; (ii) enriching the hepatitis B surface antigen protein in the homogenate with a protein-aggregating reagent to form a precipitate which contains hepatitis B surface antigen protein; (iii) dissolving the precipitate in a buffer to form a suspension; and (iv) post-homogenizing the suspension to obtain a 10% to 50% increase in yield of the hepatitis B surface antigen protein as calculated based on a yield achieved without performing the post-homogenizing step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.