Process for purifying pentostatin
US5463035A · kind A · utility
0Cited by
3References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 28, 1995 |
| Grant date | Oct 31, 1995 |
| Priority date | — |
| Expiry date | Feb 28, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H19/052
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for purifying pentostatin from a crude fermentation beer comprising the steps of 1) applying the fermentation beer to a strong cation exchange resin; 2) eluting with ammonium hydroxide; 3) concentrating the eluate; 4) applying the concentrated eluate to a strongly basic anion exchange resin charged with borate ion; 5) eluting with water; 6) applying this eluate to styrene-divinylbenzene beads prewashed with ethanol; 7) eluting the beads with water; and 8) recrysllyzing the pentostatin from water and methanol to yield a product that is greater than 99.7% pure.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.