Protein ligand binding region mapping system
US5464745A · kind A · utility
5Cited by
2References
10Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 31, 1993 |
| Grant date | Nov 7, 1995 |
| Priority date | — |
| Expiry date | Mar 31, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/14022
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for determining the nucleic acid sequence that encodes ligand binding domain on any protein encoded by a known gene includes the steps of non-specifically cleaving the coding region of the known gene, size-selecting the cleavage products, cloning the size-selected gene products into an expression vector active in a heterologous host, using a novel cloning strategy expressing the peptides produced by the clones and screening for clones that produce peptides that interact with an antibody or ligand of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.