Assay and substrate for arachidonoyl-specific phospholipase A2
US5464754A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 17, 1993 |
| Grant date | Nov 7, 1995 |
| Priority date | — |
| Expiry date | Aug 17, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/918
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A non-radioactive, spectrophotometric, microtiter plate assay for human cystolic phospholipase A.sub.2 (cPLA.sub.2) is described. The assay utilizes a novel synthetic thiol-phospholipid analog as a substrate. In one embodiment, the substrate is a phosphatidylcholine derivative with an arachidonoylthioester in the sn-2 position and an alkenyl-ether or alkenyl-ether in the sn-1 position. The alkyl-ether and the alkenyl-ether in the sn-1 position of the substrate ensures that the assay will only measure cPLA.sub.2 activity and will not be complicated by metabolism of the lysophospholipid product by the enzyme's and lysophospholypase activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.