Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5464758A · kind A · utility
Inventors
Key dates
| Filing date | Jun 14, 1993 |
| Grant date | Nov 7, 1995 |
| Priority date | — |
| Expiry date | Jun 14, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A first polynucleotide molecule coding for a transactivator fusion protein comprising the tet repressor and a protein capable of activating transcription in eucaryotes. A second polynucleotide molecule coding for a protein, wherein said polynucleotide is operably linked to a minimal promoter operably linked to at least one tet operator sequence is also disclosed. A method to regulate the expression of a protein coded for by a polynucleotide, by cultivating the eucaryotic cell of the invention in a medium comprising tetracycline or a tetracycline analogue is also disclosed. Kits containing the polynucleotide molecules are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.