Process for the enzymatic preparation of aliphatic alcohols and aldehydes from linoleic acid, linoleic acid, or a natural precursor
US5464761A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 1994 |
| Grant date | Nov 7, 1995 |
| Priority date | — |
| Expiry date | Jan 21, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The aldehydes, N-hexanal, 3-(Z)-hexen-1-al and 2-(E)-hexen-1-al, and their corresponding alcohols are enzymatically produced in high yield from linoleic, linolenic acid or a natural precursor thereof such as hydrolyzed oil. The acid or natural precursor is mixed with soy flour containing lipoxygenase which converts the acid to 13-hydroperoxy-octadeca-9,11-dienoic acid or 13-hydroperoxy-octadeca-9,11,15-trienoic acid. Guava homogenate containing lyase is added to convert the 13-hydroperoxy-octadeca-9,11-dienoic acid into n-hexanal or the 13-hydroperoxy-octadeca-9,11,15-trienoic acid into 3-(Z)-hexen-1-al. The n-hexanal or 3-(Z)-hexen-1-al is recovered, or reduced with yeast to n-hexanol or 3-(Z)-hexen-1-ol, respectively. Alternatively, the 3-(Z)-hexen-1-al is isomerized to obtain 2-(E)-hexen-1-al which is recovered or reduced with yeast to 2-(E)-hexen-1-ol which is recovered. The yeast used for reducing and isomerizing is Saccharomyces cerevisiae. Steam distillation and/or extraction with an inert organic solvent is used in recovery of the aldehydes and alcohols.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.